Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 346184
Title Reconstruction of central carbon metabolism in Sulfolobus solfataricus using a two-dimensional gel electrophoresis map, stable isotope labelling and DNA microarray analysis
Author(s) Snijders, B.P.L.; Walther, J.; Peter, S.; Kinnman, I.; Vos, M.J.G. de; Werken, H.J.G. van de; Brouns, S.J.J.; Oost, J. van der; Wright, P.C.
Source Proteomics 6 (2006)5. - ISSN 1615-9853 - p. 1518 - 1529.
Department(s) Microbiological Laboratory
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) entner-doudoroff pathway - citric-acid cycle - hyperthermophilic archaea - archaebacterium sulfolobus - citrate synthase - phosphoenolpyruvate carboxylase - thermoacidophilic crenarchaeon - thermoplasma-acidophilum - thermoproteus-tenax - glycolytic pathways
Abstract In the last decade, an increasing number of sequenced archaeal genomes have become available, opening up the possibility for functional genomic analyses. Here, we reconstructed the central carbon metabolism in the hyperthermophilic crenarchaeon Sulfolobus solfataricus (glycolysis, gluconeogenesis and tricarboxylic acid cycle) on the basis of genomic, proteomic, transcriptomic and biochemical data. A 2-DE reference map of S. solfataricus grown on glucose, consisting of 325 unique ORFs in 255 protein spots, was created to facilitate this study. The map was then used for a differential expression study based on (15)N metabolic labelling (yeast extract + tryptone-grown cells (YT) vs. glucose-grown cells (G)). In addition, the expression ratio of the genes involved in carbon metabolism was studied using DNA microarrays. Surprisingly, only 3 and 14% of the genes and proteins, respectively, involved in central carbon metabolism showed a greater than two-fold change in expression level. All results are discussed in the light of the current understanding of central carbon metabolism in S. solfataricus and will help to obtain a system-wide understanding of this organism.
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