Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 347840
Title Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene Sequences
Author(s) Speksnijder, A.G.C.L.; Kowalchuk, G.A.; Jong, S. de; Kline, E.; Stephen, J.R.; Laanbroek, H.J.
Source Applied and Environmental Microbiology 67 (2001)1. - ISSN 0099-2240 - p. 469 - 472.
DOI https://doi.org/10.1128/AEM.67.1.469-472.2001
Department(s) Biointeracties and Plant Health
Publication type Refereed Article in a scientific journal
Publication year 2001
Keyword(s) ribosomal-rna genes - polymerase chain-reaction - chimeric molecules - dna amplification - escherichia-coli - heteroduplexes - populations - coamplification - consequence - diversity
Abstract A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations. Artifact sources were polymerase errors, a mutational hot spot, and cloning of heteroduplexes and chimeras. These data may partially explain the high degree of microheterogeneity typical of sequence clusters detected in environmental clone libraries
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