Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 349351
Title Alternative approaches can greatly reduce the number of fish used for acute toxicity testing
Author(s) Hoekzema, C.C.; Murk, A.J.; Waart, B.J. van de; Hoeven, J.C.M. van der; Roode, D.F. de
Source Environmental Toxicology and Chemistry 25 (2006)5. - ISSN 0730-7268 - p. 1322 - 1325.
DOI https://doi.org/10.1897/05-293R2.1
Department(s) Sub-department of Toxicology
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) toxiciteit - chemicaliën - tests - toxicologie - testen - vissen - toxicity - chemicals - toxicology - testing - fishes - european-union - ecotoxicity - strategy - daphnia - embryo - algae
Categories Environmental Toxicology, Ecotoxicology
Abstract We first examined the uptake kinetics of Cd and Zn in the juvenile marine black sea bream (Acanthopagrus schlegeli) over a wide range of ambient Cd and Zn concentrations, and the relationships with metal accumulation (uptake rate and amount of nonexchangeable surface binding) were established for different fish tissues. Both Cd and Zn accumulation in the body increased linearly with exposure time after the initial metal surface binding. The dissolved Cd and Zn uptake rate constants were 2.64 and 6.50 L/kg/d, respectively, and the kinetics followed a first-order process. No evidence of biphasic transport was found, in contrast to the situation in freshwater fish. Viscera were the most important sites of metal uptake, and gills were the second most important sites. The black sea breams were then acclimated at different Cd or Zn concentrations from either waterborne or dietary source for one week, and the alteration of metal uptake kinetics or subcellular distribution and metallothionein (MT) induction were further quantified. The Cd body burden was enhanced up to 8.6- and 49-fold after waterborne and dietary Cd pre-exposure, respectively. Cadmium pre-exposure also altered the tissue-specific subcellular Cd distribution and significantly elevated tissue MT levels. In contrast, the black sea breams were able to regulate Zn accumulation, and waterborne or dietary Zn pre-exposure had only weak influences on Zn body burden and redistribution. Both Cd and Zn pre-exposures enhanced the metal uptake rate constants, whereas the nonexchangeable surface bindings were less impacted by these pre-exposures. We demonstrated a positive relationship between the Cd uptake rate and Cd or MT concentration in the fish. Pre-exposure to metals may substantially modify the kinetics of metal uptake.
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