Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 351090
Title Eukaryotic diversity in historical soil samples
Author(s) Moon-van der Staay, S.Y.; Tzeneva, V.A.; Staay, G.W.M. van der; Vos, W.M. de; Smidt, H.; Hackstein, J.H.P.
Source FEMS microbiology ecology 57 (2006)3. - ISSN 0168-6496 - p. 420 - 428.
Department(s) Microbiology
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) polymerase chain-reaction - gel-electrophoresis dgge - 18s rdna sequences - ribosomal-rna - molecular techniques - anoxic environments - dna - protozoa - ecology - populations
Abstract The eukaryotic biodiversity in historical air-dried samples of Dutch agricultural soil has been assessed by random sequencing of an 18S rRNA gene library and by denaturing gradient gel electrophoresis. Representatives of nearly all taxa of eukaryotic soil microbes could be identified, demonstrating that it is possible to study eukaryotic microbiota in samples from soil archives that have been stored for more than 30 years at room temperature. In a pilot study, 41 sequences were retrieved that could be assigned to fungi and a variety of aerobic and anaerobic protists such as cercozoans, ciliates, xanthophytes (stramenopiles), heteroloboseans, and amoebozoans. A PCR-denaturing gradient gel electrophoresis analysis of samples collected between 1950 and 1975 revealed significant changes in the composition of the eukaryotic microbiota.
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