Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 351944
Title Effect of Temperature and High Pressure on the Activity and Mode of Action of Fungal Pectin Methyl Esterase
Author(s) Duvetter, T.; Fraeye, I.; Sila, D.N.; Verlent, I.; Smout, C.; Clynen, E.; Schoofs, L.; Schols, H.A.; Hendrickx, M.; Loey, A. van
Source Biotechnology Progress 22 (2006)5. - ISSN 8756-7938 - p. 1313 - 1320.
DOI https://doi.org/10.1021/bp060079x
Department(s) Food Chemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) aspergillus-niger - apple pectin - pectinmethylesterase - firmness - endopolygalacturonase - methylesterase - infusion - methanol - enzyme - fruits
Abstract Pectin was de-esterified with purified recombinant Aspergillus aculeatus pectin methyl esterase (PME) during isothermal-isobaric treatments. By measuring the release of methanol as a function of treatment time, the rate of enzymatic pectin conversion was determined. Elevated temperature and pressure were found to stimulate PME activity. The highest rate of PME-catalyzed pectin de-esterification was obtained when combining pressures in the range 200-300 MPa with temperatures in the range 50-55 C. The mode of pectin de-esterification was investigated by characterizing the pectin reaction products by enzymatic fingerprinting. No significant effect of increasing pressure (300 MPa) and/or temperature (50 C) on the mode of pectin conversion was detected.
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