Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 352371
Title Detection of egg yolk antibodies reflecting Salmonella enteritidis infections using a surface plasmon resonance biosensor
Author(s) Thomas, M.E.; Bouma, A.; Eerden, E. van; Landman, W.J.M.; Knapen, F. van; Stegeman, J.A.; Bergwerff, A.A.
Source Journal of Immunological Methods 315 (2006)1-2. - ISSN 0022-1759 - p. 68 - 74.
DOI https://doi.org/10.1016/j.jim.2006.07.008
Department(s) Adaptation Physiology
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) laying hens - typhimurium - chickens - elisa - assay - immunosensor - netherlands - antigens - serum
Abstract A surface plasmon resonance (SPR) biosensor assay was developed on the basis of a lipopolysaccharide antigen of Salmonella enterica serovar enteritidis (S. enterica serovar enteritidis) to detect egg yolk antibodies against S. enterica serovar enteritidis. This biosensor assay was compared to two commercial ELISA kits based on LPS antigen and flagellar antigen. A number of 163 egg yolk and combined egg white and yolk samples from chickens experimentally infected with S. enterica serovar enteritidis and 90 egg yolk and combined egg white and yolk samples from uninfected chickens were analyzed. Receiver operating characteristic analysis of the data calculated a diagnostic sensitivity of 82% and a diagnostic specificity of 100%. The within-day coefficient of variation of a positive internal-control egg yolk was 1%. The SPR biosensor assay was able to detect antibodies in a significantly higher percentage of known positive samples than the commercial ELISA's. The anticipated use of the SPR biosensor assay is to determine the S. enterica serovar enteritidis serostatus of non-vaccinated layer hens
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