Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 354497
Title Chromogenic media for the detection and/or enumeration of Listeria monocytogenes - results of trials performed by a working group of the International Organization for Standardization - ISO/TC 34/SC 9
Author(s) Beumer, R.R.; Hazeleger, W.C.
Source Archiv für Lebensmittelhygiene 58 (2007). - ISSN 0003-925X - p. 47 - 50.
Department(s) Food Microbiology
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) food
Abstract The solid selective media PALCAM and Oxford agar originally described in the ISO (International Organization for Standardization) Standard 11290 part 1 and part 2 "Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes", suffer from the disadvan-tage that L monocytogenes cannot be differentiated from non-pathogenic liste-rias.On the basis of the results of trials, organised by an ISO/TC 34/SC 9 working group with blood-based and chromogenic media, ISO members agreed that the ALOA medium and Rapid'L.mono agar performed significantly better than the other media tested.Moreover, the ready-to-use ALOA plates performed similarly to the medium prepared from the individual components. Finally Substitution of the expensive component phosphatidylinositol (Pl), Substrate for phospholipase C activity of L monocytogenes, by lecithin had no noticeable effect on the elective features of the ALOA medium, provided the lecithin contained a sufficiently high concentration of Pl.
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