Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 355514
Title Expression of alternansucrase in potato plants
Author(s) Kok-Jacon, G.A.; Vincken, J.P.; Wang, Denong; Liu, Shaoyi; Visser, R.G.F.
Source Biotechnology Letters 29 (2007)7. - ISSN 0141-5492 - p. 1135 - 1142.
Department(s) Plant Breeding
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) leuconostoc-mesenteroides - transgenic plants - sucrose - gene - starch - biosynthesis - dextran
Abstract Alternan, which consists of alternating ¿-(1¿3)/¿-(1¿6)-linked glucosyl residues, was produced in potato tubers by expressing a mature alternansucrase (Asr) gene from Leuconostoc mesenteroides NRRL B-1355 in potato. Detection of alternan was performed by enzyme-linked immunosorbent assay in tuber juices, revealing a concentration between 0.3 and 1.2 mg g-1 fresh wt. The Asr transcript levels correlated well with alternan accumulation in tuber juices. It appeared that the expression of sucrose-regulated starch-synthesizing genes (ADP-glucose pyrophosphorylase subunit S and granule-bound starch synthase I) was down-regulated. Despite this, the physico-chemical properties of the transgenic starches were unaltered. These results are compared to those obtained with other transgenic potato plants producing mutan [¿-(1¿3)-linked glucosyl residues] and dextran [¿-(1¿6)-linked glucosyl residues].
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