Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 356587
Title The cytoplasmic domain of tomato spotted witl virus Gn glycoprotein is required for Golgi localisation and interaction with Gc
Author(s) Snippe, M.; Smeenk, L.; Goldbach, R.W.; Kormelink, R.J.M.
Source Virology 363 (2007)2. - ISSN 0042-6822 - p. 272 - 279.
DOI https://doi.org/10.1016/j.virol.2006.12.038
Department(s) Laboratory of Virology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) intracellular-transport - hantaan virus - retention - g1 - signal - coexpression - trafficking - expression - complex - cells
Abstract Envelopment of tomato spotted wilt virus nucleocapsids occurs at the Golgi stacks of infected cells. This is also the place where the two membrane glycoproteins Gn and Gc accumulate upon coexpression. The required Golgi retention signal has previously been demonstrated to reside within Gn. Using a series of truncated Gn proteins, the Golgi retention signal was mapped to a stretch of 10 amino acids on this protein's cytoplasmic tail, 20 residues downstream the transmembrane domain. Studies on the intracellular distribution of chimeric Gc proteins in which the cytoplasmic tail and/or transmembrane domain were exchanged by those from Gn, demonstrated the additional requirement of the Gn transmembrane domain for Golgi targeting. Truncated Gn constructs lacking the C-terminal 20 amino acids but still localising to the Golgi were no longer able to redirect Gc, suggesting the requirement of this domain for interaction with Gc.
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