Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 356608
Title Selective blockade of Phosphodiesterases Type 2, 5 and 9 results in cGMP accumulation in Retinal Pigment Epithelium Cells
Author(s) Diederen, R.; Heij, E.C. La; Markerink-van Ittersum, M.; Kijlstra, A.; Hendrikse, F.; Vente, J. de
Source British Journal of Ophthalmology 91 (2007)3. - ISSN 0007-1161 - p. 379 - 384.
Department(s) Wageningen Livestock Research
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) nucleotide phosphodiesterases - subretinal fluid - cgmp phosphodiesterase - rat-brain - sildenafil - cloning - pde10a - camp - amp
Abstract Aim: To investigate which phosphodiesterase (PDE) is involved in regulating cyclic 3'5' guanosine monophosphate breakdown in retinal pigment epithelium (RPE) cells. Methods: cGMP content in the cultured RPE cells (D407 cell line) was evaluated by immunocytochemistry in the presence of non-selective or isoform-selective PDE inhibitors in combination with the particulate guanylyl cyclase stimulator atrial natriuretic peptide (ANP) or the soluble guanylyl cyclase stimulator sodium nitroprusside (SNP). mRNA expression of PDE2, PDE5 and PDE9 was studied in cultured human RPE cells and rat RPE cell layers using non-radioactive in situ hybridisation. Results: In the absence of PDE inhibitors, cGMP levels in cultured RPE cells are very low. cGMP accumulation was readily detected in cultured human RPE cells after incubation with Bay60–7550 as a selective PDE2 inhibitor, sildenafil as a selective PDE5 inhibitor or Sch51866 as a selective PDE9 inhibitor. In the presence of PDE inhibition, cGMP content increased markedly after stimulation of the particulate guanylyl cyclase. mRNA of PDE2,PDE5 and PDE9 was detected in all cultured human RPE cells and also in rat RPE cell layers
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