Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 356639
Title The Chilo iridescent virus DNA polymerase promoter contains an essential AAAAT motif
Author(s) Nalcacioglu, R.; Ince, I.A.; Vlak, J.M.; Demirbag, Z.; Oers, M.M. van
Source Journal of General Virology 88 (2007)9. - ISSN 0022-1317 - p. 2488 - 2494.
DOI https://doi.org/10.1099/vir.0.82947-0
Department(s) Laboratory of Virology
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) lymphocystis disease virus - capsid protein genes - frog virus-3 - iridovirus - genome - transcription - cells - replication - baculovirus - mosquito
Abstract The delayed-early DNA polymerase promoter of Chilo iridescent virus (CIV), officially known as Invertebrate iridescent virus, was fine mapped by constructing a series of increasing deletions and by introducing point mutations. The effects of these mutations were examined in a luciferase reporter gene system using Bombyx mori cells transfected with promoter constructs and infected with CIV. When the size of the upstream element was reduced from position ¿19 to ¿15, relative to the transcriptional start site, the luciferase activity was reduced to almost zero. Point mutations showed that each of the 5 nt (AAAAT) located between ¿19 and ¿15 were equally essential for promoter activity. Mutations at individual bases around the transcription initiation site showed that the promoter extended until position ¿2 upstream of the transcription start site. South-Western analysis showed that a protein of approximately 100 kDa interacted with the ¿19 nt promoter fragment in CIV-infected cells. This binding did not occur with a point mutant that lacked promoter activity. The AAAAT motif was also found in the DNA polymerase promoter region of other iridoviruses and in other putative CIV delayed-early genes.
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