Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 356724
Title T-bet expression in the iris and spleen parallels disease expression during endotoxin-induced uveitis
Author(s) Li, B.; Yang, P.; Chu, L.; Zhou, H.; Huang, X.; Zhu, L.; Kijlstra, A.
Source Graefes Archive for Clinical and Experimental Ophthalmology 245 (2007)3. - ISSN 0721-832X - p. 407 - 413.
DOI https://doi.org/10.1007/s00417-006-0385-4
Department(s) Wageningen Livestock Research
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) transcription factor - ocular inflammation - lineage commitment - interferon-gamma - dendritic cells - ciliary body - rat - macrophages - injection - cytokines
Abstract T lymphocytes have been implicated in the development of endotoxin-induced uveitis (EIU). T-bet is a Th1 cell-specific transcription factor that is involved in differentiation and effector functions. The aim of this study was to investigate kinetics of T-bet expression at the mRNA and protein levels during EIU using real-time PCR and whole-mount immunohistochemistry. Methods A single footpad injection of 200 ¿g of lipopolysaccharide (LPS) was administered to male Wistar rats in order to induce EIU. Clinical changes were followed by slit-lamp examination. The expression of T-bet mRNA in the spleen was evaluated 0, 8, 16, 24, 48, and 96 h after LPS injection using real-time PCR. Immunohistochemistry was performed on the iris whole-mounts as well as on frozen sections of the spleen to evaluate T-bet protein expression. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on the iris whole-mounts to assay apoptotic cells. Results Uveitis was observed in all rats that received LPS. T-bet+ cells and TUNEL+ cells in the iris whole-mounts showed a similar pattern in cell number and distribution and both types of cells were observed at 8 h, significantly increased 24 h, and decreased 48 h after LPS injection. T-bet expression at both the mRNA and protein levels in spleen also paralleled ocular inflammation. It was weakly detectable after 0 h, increased after 8 h (index 1.3, T-bet+ cells OD 17.43±2.15), reached its peak after 24 h (index 4.00, OD 53.52±4.00), and decreased 48 h following LPS injection (index 1.38, OD 25.75±2.45). Conclusions The results show that T-bet expression in both the iris and the spleen, and in apoptotic cells in the iris parallel the severity of intraocular inflammation after systemic LPS administration. These results suggest that T-bet may play a significant role in the dynamics of EIU.
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