Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 357414
Title Getting there: vesicles en route for plant cytokinesis
Author(s) Ozdoba, A.
Source Wageningen University. Promotor(en): Anne Mie Emons, co-promotor(en): Andre van Lammeren. - [S.l.] : S.n. - ISBN 9789085047469 - 103
Department(s) Laboratory of Cell Biology
EPS-1
Publication type Dissertation, internally prepared
Publication year 2007
Keyword(s) planten - cellen - celbiologie - blaasjes - cytoplasma - stroming - organellen - plants - cells - cellular biology - vesicles - cytoplasm - flow - organelles
Categories Plant Cell Biology
Abstract In dividing plant cells, membranous vesicles (60-80 nm in diameter) are transported to the site where a new cell wall that separates the daughter cells is formed. In this thesis the physical parameters size and stiffness that vesicles require to reach the forming cell plate were studied. Synthetic lipid vesicles and polystyrene beads were injected into dividing cells during cytokinesis. At this stage, the cell has a structure, the phragmoplast, which contains a specific configuration of microtubules and actin filaments. Vesicles of up to 150 nm in diameter were injected and all of them moved through the phragmoplast towards the forming cell plate. The cell plate is made from the fusing vesicles and becomes the cell wall between the two daughter cells. Since smaller beads of 20 and 40 nm in diameter did not move through the phragmoplast to the cell plate, the conclusion is that not size but stiffness is a limiting parameter. In order to reach the phragmoplast, vesicles have to move from the site of injection to the cell center. This instigated the question whether hydrodynamic flow occurs in the cytoplasm of plant cells. Theoretically, with a simple lattice model, as well as experimentally, with the FRAP (fluorescence recovery after photobleaching) method, free GFP molecules in the cytoplasm of tobacco BY-2 suspension cells were shown to exhibit hydrodynamic flow: a drag of molecules caused by active molecular motor-driven transport of organelles along (bundles of) actin filaments. Such a flow distributes free cytosolic molecules in the large plant cells faster than diffusion.
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