Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 358040
Title Targeted transcript mapping for agronomic traits in potato
Author(s) Fernandez del Carmen, M.A.; Celis-Gamboa, C.; Visser, R.G.F.; Bachem, C.W.B.
Source Journal of Experimental Botany 58 (2007)11. - ISSN 0022-0957 - p. 2761 - 2774.
Department(s) Plant Breeding
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) differential gene-expression - kaurenoic acid oxidase - tuber development - solanum-tuberosum - phytochrome-b - late blight - 20-oxidase gene - linkage maps - in-vitro - gibberellin
Abstract A combination of cDNA-amplified fragment length polymorphism (AFLP) and bulked segregant analysis (BSA) was used to identify genes co-segregating with earliness of tuberization in a diploid potato population. This approach identified 37 transcript-derived fragments with a polymorphic segregation pattern between early and late tuberizing bulks. Most of the identified transcripts mapped to chromosomes 5 (19 markers) and 12 (eight markers) of the paternal map. Quantitative trait locus (QTL) mapping of tuberization time also identified earliness QTLs on these two chromosomes. A potato bacterial artificial chromosome (BAC) library was screened with four of the markers linked to the main QTL. BAC contigs containing the markers showing the highest association with the trait have been identified. One of these contigs has been anchored to chromosome 5 on an ultradense genetic map of potato, which could be used as a starting point for map-based cloning of genes associated with earliness.
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