Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 358386
Title Crystallization and preliminary crystallographic analysis of an esterese with a novel domein from the hyperthermophile Thermotoga maritima
Author(s) Sun, L.; Levisson, M.; Hendriks, S.N.A.; Akveld, T.; Kengen, S.W.M.; Dijkstra, B.W.; Oost, J. van der
Source Acta Crystallographica Section F. Structural Biology and Crystallization Communications 63 (2007)9. - ISSN 1744-3091 - p. 777 - 779.
Department(s) Microbiology
Publication type Refereed Article in a scientific journal
Publication year 2007
Abstract Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 Å resolution at 100 K using synchrotron radiation. The protein crystals belong to space group P21, with unit-cell parameters a = 47.1, b = 73.9, c = 47.4 Å, ß = 104.1°. With one dimer per asymmetric unit, the crystal volume per unit protein weight (VM) is 2.3 Å3 Da1 and the solvent content is 47%.
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