Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 359037
Title Expression of beta-defensins pBD-1 and pBD-2 along the small tract of the pig: Lack of upregulation in vivo upon Salmonella typhimurium infection
Author(s) Veldhuizen, E.J.; Dijk, A. van; Tersteeg, M.H.; Kalkhove, S.I.; Meulen, J. van der; Niewold, T.A.; Haagsman, H.P.
Source Molecular Immunology 44 (2007)4. - ISSN 0161-5890 - p. 276 - 283.
DOI https://doi.org/10.1016/j.molimm.2006.03.005
Department(s) Wageningen Livestock Research
ID - Dier en Omgeving
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) human beta-defensin-2 - molecular-cloning - innate immunity - mast-cells - peptide - gene - disease - pcr - quantitation - tissue
Abstract Defensins are antimicrobial peptides that play an important role in the innate immune response in the intestine. Up to date, only one ß-defensin (pBD-1), has been described in pig, which was found to be expressed at low levels in the intestine. We set-up a quantitative PCR method to detect the gene expression of pBD-1 and a newly discovered porcine ß-defensin, pBD-2. Expression of pBD-1 mRNA increased from the proximal to the distal part of the intestine whereas pBD-2 expression decreased. The main gene expression sites for pBD-2 were kidney and liver, whereas pBD-1 was mainly expressed in tongue. The porcine small intestinal segment perfusion (SISP) technique was used to investigate effects of Salmonella typhimurium DT104 on intestinal morphology and pBD-1 and pBD-2 mRNA levels in vivo. The early responses were studied 2, 4 and 8 h post-infection in four separate jejunal and ileal segments. Immunohistochemistry showed invasion of the mucosa by Salmonella and changes in intestinal morphology. However, no concomitant changes in expression of either pBD-1 or pBD-2 were observed. We conclude that at least two defensins are differentially expressed in the intestine of pigs, and that expression of both defensins is not altered by S. typhimurium under these conditions.
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