Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 359673
Title Enzyme-linked immunosorbent assay for the detection of bovine rennet whey powder in milk powder and buttermilk powder
Author(s) Bremer, M.G.E.G.; Kemmers-Voncken, A.; Boers, E.A.M.; Frankhuizen, R.; Haasnoot, W.
Source International Dairy Journal 18 (2008)3. - ISSN 0958-6946 - p. 294 - 302.
Department(s) RIKILT - Business Unit Safety & Health
BU Microbiological & Chemical Food Analysis
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) performance liquid-chromatography - capillary-zone-electrophoresis - kappa-casein - biosensor immunoassays - dairy-products - caseinomacropeptide - solids - glycomacropeptide - macropeptide - temperature
Abstract An inhibition enzyme-linked immunosorbent assay (ELISA) for the detection of bovine rennet whey (BRW) solids in skim milk powders (SMP) and buttermilk powders is presented. The BRW content was determined in a neutralised trichloroacetic acid sample extract by binding of the dissolved caseinomacropeptide to an enzyme-labelled anti-bovine-¿-casein monoclonal antibody. Calibration curves were constructed by analysing SMP standards with different known concentrations of BRW (0¿5.8% (w/w)).The assay has a limit of detection of 0.1% (w/w) BRW powder in SMP and has high repeatability and reliability. The ELISA reached the sensitivity required for screening to conform with European Union (EU) legislation. It is easy to use, has a short assay time and is of low cost. The successful applicability of this new screening assay was demonstrated in comparison with chromatographic methods imposed by the EU, where 60 industrial samples taken by the Dutch General Inspection Service were analysed.
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