Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 359713
Title Deletion of a Helicoverpa armigera nucleopolyhedrovirus gene encoding a virion structural protein (OR1 07) increases the budded virion titre and reduces in vivo infectivity
Author(s) Pan, X.; Long, G.; Wang, R.R.; Hou, S.; Wang, H.; Zheng, Y.; Sun, X.; Westenberg, M.; Deng, F.; Vlak, J.M.; Hu, Z.
Source Journal of General Virology 88 (2007). - ISSN 0022-1317 - p. 3307 - 3316.
Department(s) Laboratory of Virology
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) single-nucleocapsid nucleopolyhedrovirus - escherichia-coli - genome sequence - baculoviruses - cloning - virus - dna
Abstract The open reading frame Ha107 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) encodes a putative protein of 51 kDa with homologues in a few group II NPVs and a granulovirus. Ha107 was transcribed as polyadenylated transcripts in infected HzAM1 insect cells. The transcripts were initiated at two distinct locations, one upstream of Ha106 (superoxide dismutase gene, sod) and the second upstream of Ha107. By Western blot analysis, two forms of the HA107 protein were detected in infected cells, a major polypeptide of 48 kDa and a minor one of 51 kDa. Western blot and immunoelectron microscopy analyses further showed that the HA107 protein was associated with the nucleocapsids of both budded virions (BVs) and occlusion-derived virions. A Ha107 knockout virus expressing enhanced green fluorescent protein and polyhedrin was constructed using bacmid technology. A one-step virus growth curve indicated that the BV titre of the knockout virus was significantly higher than that of the parental virus and a Ha107 repair virus. Bioassays indicated that the knockout virus was able to infect third-instar H. armigera larvae; however, its median lethal dose (LD50) was significantly higher than those of the parental virus and Ha107 repair virus. These data indicate that Ha107 encodes a non-essential structural protein of HearNPV virions and that deletion of this gene increases the BV titre and LD50 of the occluded virus.
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