Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 359937
Title Influence of temperature fluctuations on Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in manure.
Author(s) Semenov, A.V.; Bruggen, A.H.C. van; Overbeek, L.S. van; Termorshuizen, A.J.; Semenov, A.M.
Source FEMS microbiology ecology 60 (2007)3. - ISSN 0168-6496 - p. 419 - 428.
Department(s) Biological Farming Systems
Biointeractions and Plant Health
Biointeracties and Plant Health
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) stalmest - escherichia coli - salmonella typhimurium - temperatuur - temperatuurresistentie - pathogenen - voedselveiligheid - gastro-enteritis - voedselvergiftiging - farmyard manure - escherichia coli - salmonella typhimurium - temperature - temperature resistance - pathogens - food safety - gastroenteritis - food poisoning - green fluorescent protein - aggregative behavior - nitrogen-content - bovine manure - united-states - amended soil - survival - growth - cattle
Categories Food Microbiology / Alternative Farming
Abstract The effects of four average temperatures (7, 16, 23 and 33°C) and daily oscillations with three amplitudes (0, ±4, ±7°C) on the survival of the enteropathogens Escherichia coli O157:H7 and Salmonella serovar Typhimurium were investigated in small microcosms. Manure was inoculated with a green fluorescent protein transformed strain of either pathogen at 107 cells g¿1 dry weight. Samples were collected immediately after inoculation, and 1 and 2 weeks after inoculation for E. coli O157:H7, and immediately and after 2 and 3 weeks for Salmonella serovar Typhimurium. Population densities were determined by dilution plating and direct counting. In addition, total bacterial CFUs were determined. Growth and survival data were fitted to a modified logistic model. Analysis of the estimated parameter values showed that E. coli O157:H7 survived for shorter periods of time and was more sensitive to competition by the native microbial community than Salmonella serovar Typhimurium. Survival of both pathogens significantly declined with increasing mean temperatures and with increasing amplitude in daily temperature oscillations. The results indicated that responses of enteropathogens to fluctuating temperatures cannot be deduced from temperature relationships determined under constant temperatures.
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