Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 364092
Title Conversion of chromosome-specific RAPDs into SCAR-based anchor markers for onion linkage maps and its application to genetic analyses inother Allium species
Author(s) Masuzaki, S.; Miyazaki, T.; McCallum, J.; Heusden, A.W. van; Kik, C.; Yamashita, K.; Tashiro, Y.
Source Scientia Horticulturae 115 (2008)4. - ISSN 0304-4238 - p. 323 - 328.
DOI https://doi.org/10.1016/j.scienta.2007.10.016
Department(s) PRI Biodiversity and Breeding
Centrum voor Genetische Bronnen Nederland
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) amplified polymorphic dna - monosomic addition lines - l. aggregatum group - cepa l. - construction - assignment - sequences - aflp - locations - reveals
Abstract Integration of previously developed Allium cepa linkage maps requires the availability of anchor markers for each of the eight chromosomes of shallot (A. cepa L. common group Aggregatum). To this end, eight RAPD markers originating from our previous research were converted into SCAR markers via cloning and sequencing of RAPD amplicons and designing of 24-mer oligonucleotide primers. Of the eight pairs of SCAR primers, seven resulted in the amplification of single bands of the original RAPDs, and the remaining primer set amplified an additional band. The results of Southern hybridization using RAPD amplicons from genomic DNA of Japanese bunching onion (Allium fistulosum L.)¿shallot monosomic addition lines indicated that five SCAR markers were single shallot chromosome-specific markers and were not detected in genomic DNA of A. fistulosum. The eight SCAR primer pairs were applied to other Allium species and exhibited three types of amplification profiles, namely RAPD amplicons observed only in shallot, in shallot and Allium vavilovii, and in several Allium species. A mapping study using 65 F2 plants generated by the selfing of one interspecific cross A. cepa × Allium roylei individual integrated the SCAR marker SAOE17500 into chromosome 5 as expected. The results of the present study show that the eight SCAR primer sets specific to shallot can facilitate the mapping in A. cepa and can also serve as anchor points between maps of different Allium species
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