Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 364588
Title Qualitative screening and quantitative determination of pesticides and contaminants in animal feed using comprehensive two-dimensional gas chomatography with time-of-flight mass spectrometry
Author(s) Lee, M.K. van der; Weg, G. van der; Traag, W.A.; Mol, J.G.J.
Source Journal of Chromatography. A, Including electrophoresis and other separation methods 1186 (2008)1-2. - ISSN 0021-9673 - p. 325 - 339.
Department(s) BU Microbiological & Chemical Food Analysis
Publication type Refereed Article in a scientific journal
Publication year 2008
Keyword(s) gc x gc - fish feed - organochlorine pesticides - extraction - residues - pressure - volumes - dioxins - fruit - pcbs
Abstract A method has been developed for the target analysis of over 100 pesticides and contaminants in a complex feed matrix. The method is based on extraction with ethyl acetate, cleanup by gel permeation chromatography (GPC) and dispersive solid-phase extraction (SPE) with primary secondary amine phase (PSA), and analysis by comprehensive two-dimensional gas chromatography with full scan time-of-flight mass spectrometric detection (GC × GC¿TOF-MS). Parameters studied during method development included a dispersive SPE cleanup step after GPC, large volume injection into the GC system and the GC × GC separation. Qualitative and quantitative performance of the GC × GC system was evaluated by analyzing spiked extracts in the range equivalent to 1¿100 ¿g/kg in feed. At levels of 50 ¿g/kg and higher, all compounds targeted for could be identified fully automatically by the software based on their mass spectra. At lower levels the hit rate decreased with the concentration. System linearity was excellent in solvent and only slightly affected by matrix (correlation coefficients r ¿ 0.995 for 90% of the compounds). Limits of quantification were in the 1¿20 ¿g/kg range for most compounds. The overall method was validated for 106 compounds at the 10 and 100 ¿g/kg level. Recoveries between 70% and 110% and RSDs below 20% were obtained for the majority of the compounds.
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