Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 371787
Title Effect of tungsten and molybdenum on growth of a syntrophic coculture of Syntrophobacter fumaroxidans and Methanospirillum hungatei
Author(s) Plugge, C.M.; Jiang, B.; Bok, F.A.M. de; Tsai, C.; Stams, A.J.M.
Source Archives of Microbiology 191 (2009)1. - ISSN 0302-8933 - p. 55 - 61.
DOI https://doi.org/10.1007/s00203-008-0428-9
Department(s) Microbiology
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2009
Keyword(s) interspecies electron-transfer - formate dehydrogenases - anaerobic-digestion - methanobacterium-thermoautotrophicum - formylmethanofuran dehydrogenase - methanococcus-vannielii - methanogenic bacteria - microbial communities - hydrogen-transfer - sp-nov
Abstract The effect of tungsten (W) and molybdenum (Mo) on the growth of Syntrophobacter fumaroxidans and Methanospirillum hungatei was studied in syntrophic cultures and the pure cultures of both the organisms. Cells that were grown syntropically were separated by Percoll density centrifugation. Measurement of hydrogenase and formate dehydrogenase levels in cell extracts of syntrophically grown cells correlated with the methane formation rates in the co-cultures. The effect of W and Mo on the activity of formate dehydrogenase was considerable in both the organisms, whereas hydrogenase activity remained relatively constant. Depletion of tungsten and/or molybdenum, however, did not affect the growth of the pure culture of S. fumaroxidans on propionate plus fumarate significantly, although the specific activities of hydrogenase and especially formate dehydrogenase were influenced by the absence of Mo and W. This indicates that the organism has a low W or Mo requirement under these conditions. Growth of M. hungatei on either formate or H(2)/CO(2) required tungsten, and molybdenum could replace tungsten to some extent. Our results suggest a more prominent role for H(2) as electron carrier in the syntrophic conversion of propionate, when the essential trace metals W and Mo for the functioning of formate dehydrogenase are depleted
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