Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 380008
Title A Kunjin replicon vector encoding granulocyte macrophage colony-stimulating factor for intra-tumoral gene therapy
Author(s) Hoang-Le, D.; Smeenk, L.; Anraku, I.; Pijlman, G.P.; Wang, X.J.; Vrij, J. de; Liu, W.J.; Le, T.T.; Schroder, W.A.; Khromykh, A.A.; Suhrbier, A.
Source Gene Therapy 16 (2009). - ISSN 0969-7128 - p. 190 - 199.
Department(s) Laboratory of Virology
Publication type Refereed Article in a scientific journal
Publication year 2009
Keyword(s) west-nile-virus - cd8 t-cells - human dendritic cells - amino-acid substitution - cytokine gene-therapy - gm-csf - ifn-alpha - in-vivo - cancer-immunotherapy - clonal expansion
Abstract We have recently developed a non-cytopathic RNA replicon-based viral vector system based on the flavivirus Kunjin. Here, we illustrate the utility of the Kunjin replicon system for gene therapy. Intra-tumoral injections of Kunjin replicon virus-like particles encoding granulocyte colony-stimulating factor were able to cure >50% of established subcutaneous CT26 colon carcinoma and B16-OVA melanomas. Regression of CT26 tumours correlated with the induction of anti-cancer CD8 T cells, and treatment of subcutaneous CT26 tumours also resulted in the regression of CT26 lung metastases. Only a few immune-based strategies are able to cure these aggressive tumours once they are of a reasonable size, illustrating the potential of this vector system for intra-tumoral gene therapy applications.
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