Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 382931
Title Application of proteases in the C-terminal modification of peptides
Author(s) Gini, F.; Eggen, I.F.; Zoelen, D.J. van; Boeriu, C.G.
Source Chimica Oggi = Chemistry today 27 (2009)2. - ISSN 1973-8250 - p. 24 - 26.
Department(s) AFSG Biobased Products
Publication type Article in professional journal
Publication year 2009
Keyword(s) alkaline protease - organic-solvents - resolution - esters
Abstract The high selectivity and the mild reaction conditions of enzymatic processes prompted their application in the synthesis of peptides, where selectivity is a feature of pivotal importance. Here we report the use of the serine protease subtilisin for the selective deprotection of C-terminal tert-butyl esters, achieved in good to quantitative yield for most of the natural amino acids. The same enzyme was active in the C-terminal amidation affording excellent yields with several peptides in the presence of a variety of amino sources. Subtilisin, finally, can also catalyse the transesterification of C-terminal primary esters. All the reactions are highly selective and neither side chains modifications nor N-terminus reactions have been observed. The endopeptidase activity, an important drawback in the use of proteases, was minimized upon extensive optimization of the reaction conditions.
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