Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 38534
Title Rapid and efficient purification method for small, hydrophobic, cationicbacteriocins: purification of lactococcin B and pediocin PA-1.
Author(s) Venema, K.; Chikindas, M.L.; Seegers, J.F.M.L.; Haandrikman, A.J.; Venema, G.; Kok, J.
Source Applied and Environmental Microbiology 63 (1997). - ISSN 0099-2240 - p. 305 - 309.
Department(s) Laboratory of Phytopathology
Publication type Refereed Article in a scientific journal
Publication year 1997
Abstract The bacteriocins lactococcin B and pediocin PA-1 were purified by ethanol precipitation, preparative isoelectric focusing, and ultrafiltration. The procedure reproducibly leads to high final yields in comparison to the generally low yields obtained by column chromatography. Specifically, during isoelectric focusing no loss of activity occurs. The method, in general, should be applicable to small, hydrophobic, cationic bacteriocins.
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