Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 387646
Title A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex
Author(s) O'Donnell, K.; Gueidan, C.; Sink, S.; Johnston, P.R.; Crous, P.W.; Glenn, A.; Riley, R.; Zitomer, N.C.; Colyer, P.; Waalwijk, C.; Lee, T. van der; Moretti, A.; Kang, S.; Kim, H.S.; Geiser, D.M.; Juba, J.H.; Baayen, R.P.; Cromey, M.G.; Bithel, S.; Sutton, D.A.; Skovgaard, K.; Ploetz, R.; Kistler, H.C.; Elliot, M.; Davis, M.; Sarver, B.A.J.
Source Fungal Genetics and Biology 46 (2009)12. - ISSN 1087-1845 - p. 936 - 948.
Department(s) Laboratory of Phytopathology
Biointeracties and Plant Health
Publication type Refereed Article in a scientific journal
Publication year 2009
Keyword(s) vegetative compatibility groups - f-sp cubense - fragment-length-polymorphisms - intergenic spacer region - genetic diversity - ribosomal dna - fungus fusarium - discontinuous distribution - phylogenetic diversity - nectria-haematococca
Abstract We constructed a two-locus database, comprising partial translation elongation factor (EF-1a) gene sequences and nearly full-length sequences of the nuclear ribosomal intergenic spacer region (IGS rDNA) for 850 isolates spanning the phylogenetic breadth of the Fusarium oxysporum species complex (FOSC). Of the 850 isolates typed, 101 EF-1a, 203 IGS rDNA, and 256 two-locus sequence types (STs) were differentiated. Analysis of the combined dataset suggests that two-thirds of the STs might be associated with a single host plant. This analysis also revealed that the 26 STs associated with human mycoses were genetically diverse, including several which appear to be nosocomial in origin. A congruence analysis, comparing partial EF-1a and IGS rDNA bootstrap consensus, identified a significant number of conflicting relationships dispersed throughout the bipartitions, suggesting that some of the IGS rDNA sequences may be non-orthologous. We also evaluated enniatin, fumonisin and moniliformin mycotoxin production in vitro within a phylogenetic framework.
There are no comments yet. You can post the first one!
Post a comment
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.