Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 388302
Title Analyses of human colonic mucus obtained by an in vivo sampling technique
Author(s) Hamer, H.M.; Jonkers, D.M.A.E.; Loof, A.; Vanhoutvin, S.A.L.W.; Troost, F.J.; Venema, K.; Kodde, A.; Koek, G.H.; Schipper, R.G.; Heerde, W.L. van; Brummer, R.J.
Source Digestive and Liver Disease 41 (2009)8. - ISSN 1590-8658 - p. 559 - 564.
Department(s) Food Chemistry
Publication type Refereed Article in a scientific journal
Publication year 2009
Keyword(s) inflammatory-bowel-disease - ulcerative-colitis - mucosal cytokine - trefoil peptide - sialic acids - filter-paper - mucins - rats - glycoproteins - resistance
Abstract Background The mucus layer is an important dynamic component of the epithelial barrier. It contains mucin glycoproteins and other compounds secreted by the intestinal epithelium, such as secretory IgA. However, a standardized in vivo sampling technique of mucus in humans is not yet available. Aim To assess the validity and feasibility of mucin and protein determinations in human colonic mucus collected under physiological conditions. Subjects and methods Triplicate colonic mucus samples were collected in 11 healthy volunteers using cytology brushes during sigmoidoscopy. As an indication of the quantity of collected mucus, total protein and mucin concentrations were determined by measuring oligosaccharide equivalents and monosaccharides. Also secretory IgA and sialic acid concentrations were determined and proteomic analysis was performed using surface enhanced laser desorption/ionization-time of flight-mass spectrometry. Results Mean values of secretory IgA and sialic acid corrected for the amount of mucus ranged from 0.16 to 1.81 g secretory IgA/mmol oligosaccharide equivalents and from 12.6 to 48.6 g sialic acid/mmol oligosaccharide equivalents. Proteomic analysis of mucus is feasible and cluster analysis showed subject specific profiles. Conclusion Using cytology brushes, human colonic mucus can be sampled and under physiological conditions. These samples could give information on the composition and quality of the mucus layer.
There are no comments yet. You can post the first one!
Post a comment
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.