Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 391779
Title Galacturonic acid catabolism in Botrytis cinerea
Author(s) Zhang, L.; Kan, J. van
Source In: Book of Abstracts 10th European Conference on Fungal Genetics, Noordwijkerhout, the Netherlands, 29 March – 1 April 2010. - - p. 93 - 93.
Event 10th European Conference on Fungal Genetics, Noordwijkerhout, the Netherlands, 2010-03-29/2010-04-01
Department(s) Laboratory of Phytopathology
EPS-2
Publication type Abstract in scientific journal or proceedings
Publication year 2010
Abstract D-galacturonic acid (GalA) is the major component of pectin, which can be degraded by plant pathogens; GalA potentially is an important carbon source for microorganisms living on decaying plant material. For bacteria, a catabolic pathway of GalA has been described, which consists of five enzymes converting GalA to pyruvate and glyceraldehyde-3-phosphate. A different catabolic pathway is proposed in filamentous fungi. In Hypocrea jecorina, GalA is converted to pyruvate and glycerol via D-galacturonate reductase, Lgalactonate dehydratase, 2-keto-3-deoxy-L-galactonate aldolase, and glycerol dehydrogenase. The Botrytis cinerea genome contains a D-galacturonate reductase gene (BcgaaA), a L-galactonate dehydratase gene (BcgaaB), and a 2-keto-3-deoxy-L-galactonate aldolase gene (BcgaaC). The three genes were cloned into a protein expression vector and the enzymatic activity determined for each gene separately. The heterologous simultaneous expression of BcgaaA, BcgaaB, and BcgaaC in an E. coli ¿uxaC mutant which cannot grow on GalA is performed to determine whether the catabolic pathway from B. cinerea can restore the growth deficiency in E.coli. Targeted gene replacement of BcgaaC or both BcgaaA and BcgaaC resulted in ¿gaaC mutants and ¿gaaAC double knock-out mutants that displayed significantly reduced growth when D-galacturonic acid was used as the sole carbon source. The mutants showed similar virulence as the wild-type stain B05.10 on tomato leaves, indicating that GalA is not the main carbon source for B. cinerea growth during infection on tomato leaves. The virulence will be tested on other pectin-rich plants and tissues
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