Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 394845
Title Mucosal delivery of a pneumococcal vaccine using lactococcus lactis affords protection against respiratory infection
Author(s) Hanniffy, S.B.; Carter, A.T.; Hitchin, E.; Wells, J.
Source The Journal of Infectious Diseases 195 (2007)2. - ISSN 0022-1899 - p. 185 - 193.
DOI https://doi.org/10.1086/509807
Department(s) Host-Microbe Interactomics
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) surface protein-a - human alveolar macrophages - cellular immune-responses - toxin fragment-c - streptococcus-pneumoniae - acid bacteria - conjugate vaccines - oral immunization - heterologous pspa - lung infection
Abstract Background - Economical and effective vaccines against Streptococcus pneumoniae (pneumococcus) are needed for implementation in poorer countries where the disease burden is highest. Here, we evaluated Lactococcus lactis intracellularly producing the pneumococcal surface protein A (PspA) as a mucosal vaccine in conferring protection against pneumococcal disease. Methods - Mice were intranasally (inl) immunized with the lactococcal vaccine. Control groups were also immunized with similar amounts of recombinant PspA administered inl or subcutaneously with alum. PspA-specific antibodies in serum samples and lung lavage fluids were measured before challenge in intraperitoneal sepsis and inl respiratory-infection models of pneumococcal disease. Results - The lactococcal vaccine afforded better protection against respiratory challenge with pneumococcus than did vaccination with purified antigen given inl or by injection with alum. This finding was associated with a shift toward a Th1-mediated immune response characterized by reduced antibody titers to the PspA antigen. In the sepsis model, the lactococcal vaccine afforded resistance to disease on a par with that obtained with the injected vaccine, demonstrating its efficacy against different forms of pneumococcal disease. Conclusion - Given the safety profile of L. lactis, there is considerable potential to develop a pneumococcal vaccine for use in humans and to broaden this approach to combat other major pathogens
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