Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 396047
Title Molecular characterization of the glucose isomerase from the thermophilic bacterium Fervidobacterium gondwanense
Author(s) Kluskens, L.D.; Zeilstra, J.B.; Geerling, A.C.M.; Vos, W.M. de; Oost, J. van der
Source Environmental Technology 31 (2010)10. - ISSN 0959-3330 - p. 1083 - 1090.
DOI https://doi.org/10.1080/09593330903486673
Department(s) Microbiology
Corporate Staff
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) d-xylose isomerase - biochemical-characterization - thermotoga-neapolitana - thermus-thermophilus - escherichia-coli - thermostability - cloning - purification - expression - fructose
Abstract The gene coding for xylose isomerase from the thermophilic bacterium Fervidobacterium gondwanense was cloned and overexpressed in Escherichia coli. The produced xylose isomerase (XylA), which closely resembles counterparts from Thermotoga maritima and T. neapolitana, was purified and characterized. It is optimally active at 70 degrees C, pH 7.3, with a specific activity of 15.0 U/mg for the interconversion of glucose to fructose. When compared with T. maritima XylA at 85 degrees C, a higher catalytic efficiency was observed. Divalent metal ions Co2+ and Mg2+ were found to enhance the thermostability
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