Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 398077
Title QTL dynamics for fruit firmness and softening around an ethyle-dependent polygalacturonase gene in apple (Malus domestica Borkh.).
Author(s) Costa, F.; Peace, C.P.; Stella, S.; Serra, S.; Musacchi, S.; Bazzani, M.; Sansavini, S.; Weg, W.E. van de
Source Journal of Experimental Botany 61 (2010)11. - ISSN 0022-0957 - p. 3029 - 3039.
DOI https://doi.org/10.1093/jxb/erq130
Department(s) Plant Breeding
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) x-domestica borkh. - 1-aminocyclopropane-1-carboxylate synthase - endopolygalacturonase gene - melting-flesh - candidate gene - melon fruit - shelf-life - expression - md-acs1 - plants
Abstract Apple fruit are well known for their storage life, although a wide range of flesh softening occurs among cultivars. Loss of firmness is genetically coordinated by the action of several cell wall enzymes, including polygalacturonase (PG) which depolymerizes cell wall pectin. By the analysis of ‘Fuji’ (Fj) and ‘Mondial Gala’ (MG), two apple cultivars characterized by a distinctive ripening behaviour, the involvement of Md-PG1 in the fruit softening process was confirmed to be ethylene dependent by its transcript being down-regulated by 1-methylcyclopropene treatment in MG and in the low ethylene-producing cultivar Fj. Comparing the PG sequence of MG and Fj, a single nucleotide polymorphism (SNP) was discovered. Segregation of the Md-PG1SNP marker within a full-sib population, obtained by crossing Fj and MG, positioned Md-PG1 in the linkage group 10 of MG, co-located with a quantitative trait locus (QTL) identified for fruit firmness in post-harvest ripening. Fruit firmness and softening analysed in different stages, from harvest to post-storage, determined a shift of the QTL from the top of this linkage group to the bottom, where Md-ACO1, a gene involved in ethylene biosynthesis in apple, is mapped. This PG–ethylene-related gene has beeen positioned in the apple genome on chromosome 10, which contains several QTLs controlling fruit firmness and softening, and the interplay among the allelotypes of the linked loci should be considered in the design of a marker-assisted selection breeding scheme for apple texture.
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