Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 398704
Title Regulatory Network Identification by Genetical Genomics: Signaling Downstream of the Arabidopsis Receptor-Like Kinase ERECTA
Author(s) Terpstra, I.R.; Snoek, L.B.; Keurentjes, J.J.B.; Peeters, A.J.M.; Ackerveken, G. van den
Source Plant Physiology 154 (2010)3. - ISSN 0032-0889 - p. 1067 - 1078.
DOI https://doi.org/10.1104/pp.110.159996
Department(s) Laboratory of Nematology
Laboratory of Genetics
Laboratory of Plant Physiology
EPS
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) wrky transcription factor - defense responses - plant development - mpk4 activation - expression - map - differentiation - resistance - protein - reconstruction
Abstract Gene expression differences between individuals within a species can be largely explained by differences in genetic background. The effect of genetic variants (alleles) of genes on expression can be studied in a multifactorial way by application of genetical genomics or expression quantitative trait locus (eQTL) mapping. In this paper we present a strategy to construct regulatory networks by application of genetical genomics in combination with transcript profiling of mutants that are disrupted in single genes. We describe the network identification downstream of the receptor-like kinase ERECTA in Arabidopsis (Arabidopsis thaliana). Extending genetical genomics on the Landsberg erecta/Cape Verde islands (Ler/Cvi) recombinant inbred population with expression profiling of monogenic mutants enabled the identification of regulatory networks in the, so far elusive, ERECTA signal transduction cascade. We provide evidence that ERECTA is the causal gene for the major hotspot for transcript regulation in the Arabidopsis Ler/Cvi recombinant inbred population. We further propose additional genetic variation between Ler and Cvi in loci of the signalling pathway downstream of ERECTA, and suggest candidate genes underlying these loci. Integration of publicly available microarray expression data of other monogenic mutants allowed us to link ERECTA to a downstream MAPK signalling cascade. Our study shows that microarray data of monogenic mutants can be effectively used in combination with genetical genomics data to enhance the identification of genetic regulatory networks.
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