Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 399060
Title Toward in vitro fertilization in Brachiaria spp.
Author(s) Dusi, D.; Alves, E.R.; Willemse, M.T.M.; Falcao, R.; Valle, C.B. do; Carneiro, V.T.C.
Source Sexual Plant Reproduction 23 (2010)3. - ISSN 0934-0882 - p. 187 - 197.
Department(s) Laboratory of Cell Biology
Publication type Refereed Article in a scientific journal
Publication year 2010
Keyword(s) pollen-tube - brizantha poaceae - chromosome-number - embryo sac - colchicine - decumbens - guidance - maize - ovule
Abstract Brachiaria are forage grasses widely cultivated in tropical areas. In vitro pollination was applied to accessions of Brachiaria spp. by placing pollen of non-dehiscent anthers on a solid medium near isolated ovaries. Viability and in vitro germination were tested in order to establish good conditions for pollen development. Comparing sexual to apomictic plants, apomictic pollen has more abortion after meiosis during the microspore stage and a lower viability and, of both types, only some plants have sufficient germination in a high sugar concentration. Using in vitro pollination with the sexual plant, the pollen tube penetrates into the nucellus and micropyle, but the embryo sac degenerates and collapses. In the apomictic B. decumbens, in vitro pollination leads to the transfer of the sperm nuclei into the egg cell and the central cell. The results are discussed according to normal fertilization and barriers in sexual and apomictic plants.
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