Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 416403
Title Tomatinase from Cladosporium fulvum is required for full virulence by degrading a-tomatine during tomato infection
Author(s) Ökmen, B.; Etalo, D.W.; Collemare, J.; Stergiopoulos, I.; Vos, R.C.H. de; Joosten, M.H.A.J.; Wit, P.J.G.M. de
Source In: Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. - Wageningen, The Netherlands : - p. 37 - 38.
Event Wageningen, The Netherlands : EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 2011-11-01/2011-11-03
Department(s) Laboratory of Phytopathology
Laboratory of Plant Physiology
BIOS Applied Metabolic Systems
Publication type Abstract in scientific journal or proceedings
Publication year 2011
Abstract PO-29 Tomatinase from Cladosporium fulvum is required for full virulence by degrading a-tomatine during tomato infection Bilal Ökmen, Desalegn Woldes Etalo, Jérôme Collemare, Ioannis Stergiopoulos, Ric CH de Vos, Matthieu HAJ Joosten and Pierre JGM De Wit a) Laboratory of Phytopathology, Wageningen University,Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands b) Centre for BioSystems Genomics, P.O. Box 98, 6700 AB Wageningen, The Netherlands c) Plant Research International Bioscience, PO Box 16, 6700 AA, Wageningen, The Netherlands Plants produce several secondary metabolites with antimicrobial activity to protect themselves against fungi. a-tomatine is a steroidal glycoalkaloid presents in tomato up to 1 mM concentration. It is hypothesized that a-tomatine forms a complex with fungal membrane sterols , resulting in pore formation and loss of membrane integrity. However, Fusarium oxysporum is able to fully infect tomato due to the secretion of tomatinase. This enzyme cleaves a-tomatine into lycotreaose and tomatidine, which are non-toxic compounds. Cladosporium fulvum is a biotrophic fungus that causes tomato leaf mold. During infection C. fulvum secretes proteins to successfully colonize the apoplast of tomato leaves. Analysis of C. fulvum genome showed the presence of a single homologue (TOM) of the F. oxysporum tomatinase gene that is predicted to be secreted. Also, in vitro biochemical assay showed that C. fulvum culture filtrate could cleaves a-tomatine into lycotreaose and tomatidine. To study the role of the TOM gene in the virulence of C. fulvum, targeted gene disruption was performed for C. fulvum TOM. In the mutants, activity of tomatinase for cleavage of a-tomatine into lycotreaose and tomatidine was abolished in vitro. Also, spores germination assay showed that ¿-tom mutants were more sensitive to a-tomatine than the wild type. Tomato plants infected with the ¿-tom mutants showed a delay in the disease process, indicating that tom gene is required for the full virulence of C. fulvum. Our results indicate that detoxification of a-tomatine by C. fulvum is required for full virulence on tomato.
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