Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 432396
Title A thermophile under pressure: Transcriptional analysis of the response of Caldicellulosiruptor saccharolyticus to different H2 partial pressures
Author(s) Bielen, A.A.M.; Verhaart, M.R.A.; Vanfossen, A.L.; Blumer-Schuette, S.E.; Stams, A.J.M.; Oost, J. van der; Kelly, R.M.; Kengen, S.W.M.
Source International Journal of Hydrogen Energy 38 (2013)4. - ISSN 0360-3199 - p. 1837 - 1849.
DOI https://doi.org/10.1016/j.ijhydene.2012.11.082
Department(s) Microbiology
VLAG
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) rex-family repressor - hydrogen-production - extreme thermophiles - thermoanaerobacter-ethanolicus - hyperthermophilic archaeon - ferredoxin oxidoreductase - cellulolytic bacterium - thermotoga-neapolitana - alcohol dehydrogenases - pyrococcus-furiosus
Abstract Increased hydrogen (H2) levels are known to inhibit H2 formation in Caldicellulosiruptor saccharolyticus. To investigate this organism's strategy for dealing with elevated H2 levels the effect of the hydrogen partial pressure (PH2) on fermentation performance was studied by growing cultures under high and low PH2 in a glucose limited chemostat setup. Transcriptome analysis revealed the upregulation of genes involved in the disposal of reducing equivalents under high PH2, like lactate dehydrogenase and alcohol dehydrogenase as well as the NADH-dependent and ferredoxin-dependent hydrogenases. These findings are in line with the observed shift in fermentation profiles from acetate production to the production of acetate, lactate and ethanol under high PH2. Moreover, differential transcription was observed for genes involved in carbon metabolism, fatty acid biosynthesis and several transport systems. In addition, presented transcription data provide evidence for the involvement of the redox sensing Rex protein in gene regulation under high PH2 cultivation conditions
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