Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 437467
Title Agaricus bisporus and Agaricus brasiliensis (1 ¿ 6)-ß-d-glucans show immunostimulatory activity on human THP-1 derived macrophages
Author(s) Smiderle, F.R.; Alquini, G.; Tadra-Sfeir, M.Z.; Lacomini, M.; Wichers, H.J.; Griensven, L.J.L.D. van
Source Carbohydrate Polymers 94 (2013)1. - ISSN 0144-8617 - p. 91 - 99.
Department(s) Food Chemistry
FBR Fresh Supply Chains
BIOS Applied Metabolic Systems
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) beta-glucans - fungal metabolites - molecular-weight - polysaccharides - lipopolysaccharide - mushroom - activation - receptor - rats - expression
Abstract The (1 ¿ 6)-ß-d-glucans from Agaricus bisporus and Agaricus brasiliensis were purified to evaluate their effects on the innate immune system. THP-1 macrophages were used to investigate the induction of the expression of TNF-a, IL1ß, and COX-2 by RT-PCR. The purification of the polysaccharides gave rise to fractions containing 96–98% of glucose. The samples were analyzed by GC–MS, HPSEC and 13C NMR, which confirmed the presence of homogeneous (1 ¿ 6)-ß-d-glucans. The ß-glucans were incubated with THP-1 derived macrophages, for 3 h and 6 h to evaluate their effects on the expression of pro-inflammatory genes. Both ß-glucans stimulated the expression of such genes as much as the pro-inflammatory control (LPS). When the cells were incubated with LPS + ß-glucan, a significant inhibition of the expression of IL-1ß and COX-2 was observed for both treatments after 3 h of incubation. By the results, we conclude that the (1 ¿ 6)-ß-d-glucans present an immunostimulatory activity when administered to THP-1 derived macrophages
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