Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 437601
Title Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up
Author(s) Villaverde, J.J.; Vlist, V. van der; Santos, S.A.O.; Haarmann, T.; Langfelder, K.; Pirttimaa, M.; Nyyssola, A.; Jylhä, S.; Tamminen, T.; Kruus, K.; Graaff, L.H. de; Pascoal Neto, C.; Simoes, M.M.Q.; Domingues, M.R.M.; Silvestre, A.J.D.; Eidner, J.; Buchert, J.
Source Chemical Engineering Journal 217 (2013). - ISSN 1385-8947 - p. 82 - 90.
Department(s) Bioprocess Engineering
Systems and Synthetic Biology
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) unsaturated fatty-acids - soybean lipoxygenase - immobilized lipoxygenase - manganese lipoxygenase - mass-spectrometry - oxidation - identification - system - state - oil
Abstract Linoleic acid was converted into hydroperoxides by a Gaeumannomyces graminis tritici lipoxygenase produced recombinantly in Trichoderma reesei. Hydroperoxide production was optimized using a face-centred experimental design in order to study the effects of pH, temperature and time on the conversion of linoleic acid into four regioisomeric hydroperoxyoctadecadienoic acids (HPODE): 13-(Z,E)-, 9-(E,Z)-, 13-(E,E)-, 9-(E,E)-HPODE. Fitting equations described satisfactorily the system behavior and showed that reaction time was the most influencing independent variable. A set of independent variables (pH = 6.7, temperature = 23.9 °C and time = 18 h) allowed to obtain high yields of hydroperoxides (88.0%) with a good selectivity for the 13-(Z,E)-HPODE isomer (47.4%) when the initial substrate concentration was 10 g/L. The production was further investigated using industrially relevant linoleic acid concentrations (100–300 g/L) leading to HPODE yields of ~40% and the volumetric productivity 3.6 g/(L h), and a selectivity for 13-(Z,E)-HPODE of around 74%
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