Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 440679
Title A bead-based suspension array for the serological detection of Trichinella in pigs
Author(s) Wal, F.J. van der; Achterberg, R.P.; Kant, A.; Maassen, C.B.M.
Source The Veterinary Journal 196 (2013)3. - ISSN 1090-0233 - p. 439 - 444.
DOI https://doi.org/10.1016/j.tvjl.2012.10.029
Department(s) Infection Biology
Bacteriology & Epidemiology
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) linked-immunosorbent-assay - multiplexed luminex assay - nonstructural proteins - antibodies - trichinosis - virus - spiralis - animals - serodiagnosis - immunoassay
Abstract The feasibility of using bead-based suspension arrays to detect serological evidence of Trichinella in pigs was assessed. Trichinella spiralis excretory–secretory antigen was covalently coupled to paramagnetic beads and used to bind serum antibodies, which were subsequently detected using anti-swine antibody. The assay was evaluated by testing pig sera from farms where trichinellosis was endemic and comparing the results with those obtained using two commercially available ELISAs. With cut-offs established by receiver operating characteristic (ROC) analysis, digestion-negative sera from a Trichinella-free population of pigs were deemed seronegative. When anti-swine antibody was replaced with protein A/G, higher test sensitivity (94% vs. 88%) at similar test specificity (95%), was achieved. The potential use of this assay in species other than swine was also demonstrated by testing human sera.
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