Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 441305
Title Does acute tryptophan depletion affect peripheral serotonin metabolism in the intestine?
Author(s) Keszthelyi, D.; Troost, F.J.; Jonkers, D.M.; Donkelaar, E.L. van; Dekker, J.; Buurman, W.A.; Masclee, A.A.
Source American Journal of Clinical Nutrition 95 (2012)3. - ISSN 0002-9165 - p. 603 - 608.
DOI https://doi.org/10.3945/ajcn.111.028589
Department(s) Department of Animal Sciences
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) 5-hydroxyindoleacetic acid - gastrointestinal-tract - cerebrospinal-fluid - brain - permeability - depression - cortisol - reuptake - humans - lumbar
Abstract Background: Serotonin (5-hydroxytryptamine; 5-HT), a tryptophan metabolite, plays an important regulatory role in the human central nervous system and in the gastrointestinal tract. Acute tryptophan depletion (ATD) is currently the most widely established method to investigate 5-HT metabolism. Objective: The aim of this study was to assess the effect of an acute decrease in the systemic availability of tryptophan on intestinal 5-HT metabolism and permeability. Design: Thirty-three healthy volunteers (17 with ATD, 3 of whom dropped out; 16 placebo) participated in this randomized placebo-controlled study. Plasma and duodenal mucosal concentrations of 5-HT, 5-hydroxyindoleacetic acid (5-HIAA), and kynurenic acid (KA) were measured by HPLC-mass spectrometry. Intestinal barrier function was assessed with a multisugar plasma test, and analysis of tight junction transcription was performed in duodenal biopsy samples obtained by gastroduodenoscopy. Results: Mucosal 5-HT, 5-HIAA, and KA concentrations remained unaltered by ATD. In contrast, ATD significantly decreased plasma 5-HT (P <0.05) and 5-HIAA (P <0.0001) concentrations. After endoscopy, a significant increase in plasma 5-HT concentrations was observed in the placebo group (P = 0.029) compared with the ATD group. Moreover, a significant increase in plasma KA concentrations over time was found in the placebo group (P <0.05). No changes in intestinal barrier function were observed. Conclusions: An acute decrease in precursor availability does not affect mucosal concentrations of serotonergic metabolites, in contrast with systemic concentrations. ATD alters biochemical responses to acute stress from the endoscopic examination reflected by lower 5-HT concentrations. Changes in 5-HT concentrations were paralleled by alterations in KA concentrations, which suggest competition between the 2 metabolic pathways for the mutual precursor. This trial was registered at clinicaltrials.gov as NCT00731003. Am J Clin Nutr 2012;95:603-8.
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