Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 442727
Title Dipeptide synthesis in near-anhydrous organic media: Long-term stability and reusability of immobilized Alcalase
Author(s) Vossenberg, P.; Beeftink, H.H.; Nuijens, T.; Quaedflieg, P.J.L.M.; Cohen Stuart, M.A.; Tramper, J.
Source Journal of Molecular Catalysis. B, Enzymatic 93 (2013). - ISSN 1381-1177 - p. 23 - 27.
Department(s) Bioprocess Engineering
Physical Chemistry and Colloid Science
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) high initial activity - enzymatic esterification - subtilisin carlsberg - peptide-synthesis - shear - solvents - enzymes - deactivation - kinetics - water
Abstract The long-term stability and re-use of Alcalase covalently immobilized onto macroporous acrylic beads (Cov) in tetrahydrofuran (THF) were investigated. Cov can be used to synthesize dipeptides under near-anhydrous conditions in THF. Cov was incubated with and without molecular sieves (beads or powder) in THF, in order to investigate whether its stability is affected by the presence of molecular sieves. After different incubation periods, the enzyme activity was determined in an aqueous environment. In addition, Cov was repeatedly recycled to examine its reusability. Without molecular sieve beads, Cov hardly inactivated in THF. With molecular sieve beads, Cov lost activity over time. Incubated Cov samples were rotated on a blood rotator, entailing mechanical forces between Cov and the molecular sieve beads. Mechanical damage of Cov by the molecular sieve beads was found to be the main reason for the instability of Cov. During reuse, intermediate rehydration of Cov also caused a small but significant activity loss.
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