Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 443313
Title Chaperones of the endoplasmic reticulum are required for Ve1-mediated resistance to Verticillium
Author(s) Liebrand, T.W.H.; Kombrink, A.; Zhang, Z.; Sklenar, J.; Jones, A.M.E.; Robatzek, S.; Thomma, B.P.H.J.; Joosten, M.H.A.J.
Source Molecular Plant Pathology 15 (2014)1. - ISSN 1464-6722 - p. 109 - 117.
DOI https://doi.org/10.1111/mpp.12071
Department(s) Laboratory of Phytopathology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) receptor-like proteins - defective brassinosteroid receptor - pattern-recognition receptors - er quality-control - plant innate immunity - cell-surface - tomato ve1 - arabidopsis - gene - perception
Abstract The tomato receptor-like protein (RLP) Ve1 mediates resistance to the vascular fungal pathogen Verticillium dahliae. To identify the proteins required for Ve1 function, we transiently expressed and immunopurified functional Ve1-enhanced green fluorescent protein (eGFP) from Nicotiana benthamiana leaves, followed by mass spectrometry. This resulted in the identification of peptides originating from the endoplasmic reticulum (ER)-resident chaperones HSP70 binding proteins (BiPs) and a lectin-type calreticulin (CRT). Knock-down of the different BiPs and CRTs in tomato resulted in compromised Ve1-mediated resistance to V.dahliae in most cases, showing that these chaperones play an important role in Ve1 functionality. Recently, it has been shown that one particular CRT is required for the biogenesis of the RLP-type Cladosporium fulvum resistance protein Cf-4 of tomato, as silencing of CRT3a resulted in a reduced pool of complex glycosylated Cf-4 protein. In contrast, knock-down of the various CRTs in N.benthamiana or N.tabacum did not result in reduced accumulation of mature complex glycosylated Ve1 protein. Together, this study shows that the BiP and CRT ER chaperones differentially contribute to Cf-4- and Ve1-mediated immunity.
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