Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 445837
Title Valencene synthase from the heartwood of Nootka cypress (Callitropsis nootkatensis) for biotechnological production of valencene
Author(s) Beekwilder, M.J.; Houwelingen, A.M.M.L. van; Cankar, K.; Dijk, A.D.J. van; Jong, R. de; Stoopen, G.M.; Bouwmeester, H.J.; Achkar, J.; Sonke, Th.; Bosch, H.J.
Source Plant Biotechnology Journal 12 (2014)2. - ISSN 1467-7644 - p. 174 - 182.
DOI https://doi.org/10.1111/pbi.12124
Department(s) BIOS Applied Metabolic Systems
Laboratory of Plant Physiology
Mathematical and Statistical Methods - Biometris
BIOS Applied Bioinformatics
BU Toxicology, Novel Foods & Agrochains
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) antimalarial agent artemisinin - functional-characterization - phytophthora-ramorum - ixodes-scapularis - terpene synthases - essential oil - biosynthesis - yeast - metabolism - expression
Abstract Nootkatone is one of the major terpenes in the heartwood of the Nootka cypress Callitropsis nootkatensis. It is an oxidized sesquiterpene, which has been postulated to be derived from valencene. Both valencene and nootkatone are used for flavouring citrus beverages and are considered among the most valuable terpenes used at commercial scale. Functional evaluation of putative terpene synthase genes sourced by large-scale EST sequencing from Nootka cypress wood revealed a valencene synthase gene (CnVS). CnVS expression in different tissues from the tree correlates well with nootkatone content, suggesting that CnVS represents the first dedicated gene in the nootkatone biosynthetic pathway in C. nootkatensis The gene belongs to the gymnosperm-specific TPS-d subfamily of terpenes synthases and its protein sequence has low similarity to known citrus valencene synthases. In vitro, CnVS displays high robustness under different pH and temperature regimes, potentially beneficial properties for application in different host and physiological conditions. Biotechnological production of sesquiterpenes has been shown to be feasible, but productivity of microbial strains expressing valencene synthase from Citrus is low, indicating that optimization of valencene synthase activity is needed. Indeed, expression of CnVS in Saccharomyces cerevisiae indicated potential for higher yields. In an optimized Rhodobacter sphaeroides strain, expression of CnVS increased valencene yields 14-fold to 352 mg/L, bringing production to levels with industrial potential
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