Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 445911
Title The brassinosteroid insensitive1-like3 signalosome complex regulates Arabidopsis root development
Author(s) Fàbregas, N.; Li, N.; Boeren, S.; Nash, T.E.; Goshe, M.B.; Clouse, S.D.; Vries, S.C. de; Caño-Delgado, A.I.
Source The Plant Cell 25 (2013)9. - ISSN 1040-4651 - p. 3377 - 3388.
DOI https://doi.org/10.1105/tpc.113.114462
Department(s) Biochemistry
EPS-1
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) receptor kinase bri1 - signaling pathways - statistical-model - plasma-membrane - gene-expression - transduction - growth - identification - thaliana - network
Abstract Brassinosteroid (BR) hormones are primarily perceived at the cell surface by the leucine-rich repeat receptor-like kinase brassinosteroid insensitive1 (BRI1). In Arabidopsis thaliana, BRI1 has two close homologs, BRI1-LIKE1 (BRL1) and BRL3, respectively, which are expressed in the vascular tissues and regulate shoot vascular development. Here, we identify novel components of the BRL3 receptor complex in planta by immunoprecipitation and mass spectrometry analysis. Whereas BRI1 associated kinase1 (BAK1) and several other known BRI1 interactors coimmunoprecipitated with BRL3, no evidence was found of a direct interaction between BRI1 and BRL3. In addition, we confirmed that BAK1 interacts with the BRL1 receptor by coimmunoprecipitation and fluorescence microscopy analysis. Importantly, genetic analysis of brl1 brl3 bak1-3 triple mutants revealed that BAK1, BRL1, and BRL3 signaling modulate root growth and development by contributing to the cellular activities of provascular and quiescent center cells. This provides functional relevance to the observed protein-protein interactions of the BRL3 signalosome. Overall, our study demonstrates that cell-specific BR receptor complexes can be assembled to perform different cellular activities during plant root growth, while highlighting that immunoprecipitation of leucine-rich repeat receptor kinases in plants is a powerful approach for unveiling signaling mechanisms with cellular resolution in plant development
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