Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 449386
Title Fine mapping quantitative resistances to downy mildew in lettuce revealed multiple sub-QTLs with plant stage dependent effects reducing or even promoting the infection
Author(s) Boer, E. den; Zhang, N.; Pelgrom, K.T.B.; Visser, R.G.F.; Niks, R.E.; Jeuken, M.J.W.
Source Theoretical and Applied Genetics 126 (2013)12. - ISSN 0040-5752 - p. 2995 - 3007.
DOI https://doi.org/10.1007/s00122-013-2188-4
Department(s) Plant Breeding
Plant Breeding
PBR Non host and insect resistance
EPS
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) backcross inbred lines - trait locus qtl - bremia-lactucae - leaf rust - durable resistance - stripe rust - genetic dissection - disease resistance - nonhost resistance - wild lettuce
Abstract Previous studies on the genetic dissection of the complete resistance of wild lettuce, Lactuca saligna, to downy mildew revealed 15 introgression regions that conferred plant stage dependent quantitative resistances (QTLs). Three backcross inbred lines (BILs), carrying an individual 30–50 cM long introgression segment from L. saligna in a cultivated lettuce, L. sativa, background, reduced infection by 60–70 % at young plant stage and by 30–50 % at adult plant stage in field situations. We studied these three quantitative resistances in order to narrow down their mapping interval and determine their number of loci, either single or multiple. We performed recombinant screenings and developed near isogenic lines (NILs) with smaller overlapping L. saligna introgressions (substitution mapping). In segregating introgression line populations, recombination was suppressed up to 17-fold compared to the original L. saligna × L. sativaF2 population. Recombination suppression depended on the chromosome region and was stronger suppressed at the smallest introgression lengths. Disease evaluation of the NILs revealed that the resistance of all three BILs was not explained by a single locus but by multiple sub-QTLs. The 17 L. saligna-derived sub-QTLs had a smaller and plant stage dependent resistance effect, some segments reducing; others even promoting downy mildew infection. Implications for lettuce breeding are outlined.
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