Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 451888
Title Analysis of a Natural Yellow Dye: An Experiment for Analytical Organic Chemistry
Author(s) Villela, A.; Derksen, G.C.H.; Beek, T.A. van
Source Journal of Chemical Education 91 (2014)4. - ISSN 0021-9584 - p. 566 - 569.
DOI https://doi.org/10.1021/ed400331f
Department(s) VLAG
Laboratory of Entomology
Organic Chemistry
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) weld
Abstract This experiment exposes second-year undergraduate students taking a course in analytical organic chemistry to high-performance liquid chromatography (HPLC) and quantitative analysis using the internal standard method. This is accomplished using the real-world application of natural dyes for textiles. The extracted flavonoids of the plant weld are responsible for the yellow color of the dyed wool. Dried and ground weld is extracted for dyeing wool and quantifying the plant’s three main flavonoids. The students also mimic the work of chemists investigating historical textiles by carrying out a small-scale extraction of the dyed wool. Twenty-one students carried out the experiment, and their samples were analyzed using either a traditional 5 µm-particle size HPLC column or a modern 1.8 µm-particle size ultrahigh-pressure liquid chromatography (UHPLC) column mounted in a conventional HPLC system.
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