Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 454706
Title Assessing the immunomodulatory potential of high-molecular-weight extracts from mushrooms; an assay based on THP-1 macrophages
Author(s) Velde, J. van de; Wilbers, R.H.P.; Westerhof, L.B.; Raaij, D.R. van; Stavrakaki, I.; Sonnenberg, A.S.M.; Bakker, J.; Schots, A.
Source Journal of the Science of Food and Agriculture 95 (2015)2. - ISSN 0022-5142 - p. 344 - 350.
DOI https://doi.org/10.1002/jsfa.6726
Department(s) Laboratory of Nematology
Plant Breeding
Laboratorium voor Monoklonale Antistoffen
EPS
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) monocytic leukemia-cells - agaricus-blazei murill - toll-like receptors - in-vitro - induction - responses - line - polysaccharides - differentiation - expression
Abstract BACKGROUND Food is a potential source of immunomodulating compounds that may be used to steer immune responses towards a desired status such as reducing inflammatory disorders. However, to identify and characterize such bioactive compounds, biologically relevant and standardized assays are required. Macrophages play an important role in immunomodulation and are suited for developing cell-based assays. An assay was developed based on macrophages, in a homogeneous differentiation state, using the human monocytic cell line THP-1 previously used to assess immunomodulatory properties of low-molecular-weight allergens, hormones, dietary supplements and therapeutic drugs. RESULTS Zymosan and mushroom polysaccharide extracts lead to a heterogeneous differentiation state of THP-1 monocytes, and these cells secrete low levels of cytokines upon stimulation. Differentiation into macrophages using a low concentration of phorbol 12-myristate 13-acetate improved responsiveness. Elevated levels of cytokines were secreted by cells in a homogenous differentiation state. In addition, it was determined that the assay performs best when using cells at a concentration of (2.5–5)¿×¿105 cells mL-1. CONCLUSION An assay was developed suitable to distinguish the immunomodulatory properties of food compounds in a reproducible manner. It was evaluated using eight mushroom species by measuring the secretion of relevant cytokines TNF-a, IL-1ß, IL-6 and IL-10. © 2014 Society of Chemical Industry
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