AMP-dependent protein kinase (AMPK) is a phylogenetically conserved enzyme that senses changes in the AMP/ATP ratio in cells and, consequently, acts as a “metabolic master switch” mediating the cellular adaptation to environmental or nutritional stress factors. In mammals, AMPK is activated in skeletal muscle by exercise, resulting in increased GLUT4-mediated glucose uptake and utilization. However, little is known regarding the potential mediatory role of AMPK on the metabolic effects of swimming in fish. Our group has recently begun to investigate the metabolic role of AMPK and its regulation by contractile activity in skeletal muscle in fish. Our results indicate that AMPK stimulates the uptake and utilization of glucose in fish skeletal muscle cells by specifically up-regulating the GLUT4-dependent glucose transport machinery as well as the mRNA levels of genes involved in glucose utilization. Importantly, we have obtained evidence for the stimulatory effect of muscle contractile activity, either induced in vivo by swimming or in vitro by electrical stimulation of trout myotubes, on AMPK activity. Therefore, AMPK appears to be an important metabolic sensor in fish skeletal muscle. CAN MARINE FISHERY BY-PRODUCTS REDUCE STRESS RESPONSE IN CULTURED ATLANTIC SALMON? Plante, S.,
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