Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 455548
Title Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purifiaction
Author(s) Kozarski, M.; Klaus, A.; Jakovljevic, D.; Todorovic, N.; Niksic, M.; Vrvic, M.M.; Griensven, L.J.L.D. van
Source Food Research International 64 (2014). - ISSN 0963-9969 - p. 53 - 64.
DOI https://doi.org/10.1016/j.foodres.2014.05.075
Department(s) BIOS Applied Metabolic Systems
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) pleurotus-sajor-caju - antioxidative activities - edible mushroom - beta-glucan - medicinal mushrooms - antitumor-activity - phellinus-linteus - blazei murill - cell-wall - saccharomyces-cerevisiae
Abstract Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 ¿ 6)-ß-D-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-¿ inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by ß-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-¿ inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 µg/mL the cells’ viabilitywas decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against •OH and •O2- radicals of HWPE I and PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against •OH radicals in the Fenton systemwas achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.
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