Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 455751
Title Distance Measurements on Orthogonally Spin-Labeled Membrane Spanning WALP23 Polypeptides
Author(s) Lueders, P.; Jäger, H.; Hemminga, M.A.; Jeschke, G.; Yulikov, M.
Source The Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical 117 (2013)7. - ISSN 1520-6106 - p. 2061 - 2068.
Department(s) Biophysics
Publication type Refereed Article in a scientific journal
Publication year 2013
Keyword(s) electron double-resonance - trichogin ga-iv - alpha-helical peptides - dipole-dipole interactions - islet amyloid polypeptide - field pulsed epr - hydrophobic mismatch - phospholipid membrane - gd3+ complexes - spectroscopy
Abstract EPR-based Gd(III)-nitroxide distance measurements were performed on a series of membrane-incorporated orthogonally labeled WALP23 polypeptides. The obtained distance distributions were stable upon the change of detection frequency from 10 GHz (X-band) to 35 GHz (Q-band). The alpha-helical pitch of WALP23 polypeptide could be experimentally observed, despite the flexibility of the two spin labels. The spectroscopic properties of Gd(III) ions and nitroxide radicals allow detecting both types of paramagnetic species selectively in different EPR experiments. In particular, this spectroscopic selectivity allows for supplementing Gd(III)-nitroxide distance measurements with independent checks of polypeptide aggregation and with measurements of the local environment of the nitroxide spin labels. All mentioned additional checks do not require preparation of further samples, as it is the case in the experiments with pairs of identical nitroxide spin labels.
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