Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 455858
Title Suitability of faeces and tissue samples as a basis for non-invasive sampling for African swine fever in wild boar
Author(s) Carvalho Ferreira, H.C. de; Weesendorp, E.; Quak, S.; Stegeman, J.A.; Loeffen, W.L.A.
Source Veterinary Microbiology 172 (2014)3-4. - ISSN 0378-1135 - p. 449 - 454.
DOI https://doi.org/10.1016/j.vetmic.2014.06.016
Department(s) Infection Biology
Virology
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) terrestrial ecosystems - sus-scrofa - virus - size - europe - pigs
Abstract A challenging aspect of ASFV control in wild boar populations is the design and implementation of effective surveillance and monitoring programmes, both for early warning, and to determine the ongoing epidemiological situation in an infected population. Testing blood samples requires invasive sampling strategies like hunting or capture of wild boar. Besides being biased towards healthy animals, such strategies are also linked to further spread of the virus. Non-invasive sampling strategies would increase the reliability of surveillance of ASFV in wild boar populations, without the negative side effects. This study evaluates the potential of faeces and tissue samples as a basis for non-invasive sampling strategies for ASFV in wild boar. In the acute phase (0–21 days after infection), in comparison with virus detection in blood, virus can be detected in faeces 50–80% of the time. This percentage decreases to below 10% for the subacute/chronic phase. ASFV DNA is quite stable in faeces. Half-lives range from more than 2 years at temperature up to 12 °C, to roughly 15 days at temperatures of 30 °C. In tissue samples, stored at 20 °C, half-lives mostly range from 1.7 to 7.4 days. The sample of preference is the spleen, where the highest titres and highest half-life of ASFV DNA are observed. The level and duration of excretion of ASFV in the faeces, combined with the stability of the DNA, suggest that sampling of faeces could be the basis for a non-invasive sampling strategy to monitor ASFV in wild boar.
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